The match will be played at Bedfont Sport FC, TW14 9QT, kick-off 3pm. Tickets are limited to 400 on the day and priced at £3 for over-16s.
Karleigh Osborne's side progressed through the Extra-Preliminary Round with a penalty shootout victory over Royal Wootton Bassett WFC. Following a 1-1 draw in normal time, The Bees went through 7-6 on penalties against their higher division opponents.
Ashford play in the Premier Division of the London and South East Regional Women's League, two leagues above The Bees. The Tangerines opened their season with a 2-0 defeat at Dartford on Sunday.